serum ykl 40 levels  (Boster Bio)

Journal: Biomolecules

Article Title: YKL-40, Soluble IL-2 Receptor, Angiotensin Converting Enzyme and C-Reactive Protein: Comparison of Markers of Sarcoidosis Activity

doi: 10.3390/biom8030084

Figure Lengend Snippet: Biochemical parameters in control, active and inactive sarcoidosis patients, ( A ) High-sensitivity C-reactive protein (hs-CRP) (mg/L); ( B ) Neopterin (NEO) (ng/mL); ( C ) Adenosine deaminase (ADA) (U/L); ( D ) Chitinase-3-Like Protein 1 (YKL–40) (ng/mL); ( E ) Soluble interleukin-2 receptor (sIL-2R) (ng/mL); ( F ) Angiotensin-converting enzyme ACE (ng/mL). a: vs. control; b: vs. inactive sarcoidosis. * p < 0.05; ** p < 0.01; *** p ≤ 0.001.

Article Snippet: The serum YKL-40 levels were assayed by a sandwich immunoassay in a microtitre stripwell format (Chondrex, Metra Biosystems Inc., Mountainview, CA, USA).

Techniques:

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: Chitinase 3-like 1-CD44 interaction promotes metastasis and epithelial-to-mesenchymal transition through β-catenin/Erk/Akt signaling in gastric cancer

doi: 10.1186/s13046-018-0876-2

Figure Lengend Snippet: Upregulation of CHI3L1 correlates with distant metastasis and poor prognosis of human GCs. a Western blot analysis of CHI3L1 protein expression in 5 pairs of gastric tissues (GC and adjacent non-cancerous tissues) after curative resection. Data represent mean ± SEM; * p < 0.05 (Student’s t test). b Immunohistochemical analysis of CHI3L1 protein levels in GC samples on tissue microarrays. Representative examples of CHI3L1 expression in adjacent non-cancerous gastric tissues, GC tissues and GC metastases tissues (lymph node and pancreas) are shown. Scale bars represent 100 μm. c - e Serum CHI3L1 levels in GC patients ( n = 100) and normal controls ( n = 50) ( c ); Serum CHI3L1 levels in GC patients with different tumor invasive depth ( d ) ( n = 17 [T1 + T2], n = 83 [T3 + T4]) and lymph node metastasis ( e ) ( n = 21 [N0 + N1], n = 79 [N1 + N2]). Mann-Whitney U test was used to assess p values; * p < 0.05. f - i The overall survival of patients with high or low CHI3L1 expression in GC tissues. Kaplan-Meier test was used to analyze p values

Article Snippet: Human serum CHI3L1 levels (R&D Systems; Cat. #DC3 L10) and total (R&D Systems; Cat. #MB100B) and active (Biolegend, San Diego, CA; Cat. #437707) mouse TGF-β1 levels in mouse broncho-alveolar lavage fluid were measured using ELISA kits as directed by the manufacturer.

Techniques: Western Blot, Expressing, Immunohistochemical staining, MANN-WHITNEY

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: Chitinase 3-like 1-CD44 interaction promotes metastasis and epithelial-to-mesenchymal transition through β-catenin/Erk/Akt signaling in gastric cancer

doi: 10.1186/s13046-018-0876-2

Figure Lengend Snippet: Associations between CHI3L1 Expression and Clinical Pathological Characteristics in Patients with GC

Article Snippet: Human serum CHI3L1 levels (R&D Systems; Cat. #DC3 L10) and total (R&D Systems; Cat. #MB100B) and active (Biolegend, San Diego, CA; Cat. #437707) mouse TGF-β1 levels in mouse broncho-alveolar lavage fluid were measured using ELISA kits as directed by the manufacturer.

Techniques: Expressing

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: Chitinase 3-like 1-CD44 interaction promotes metastasis and epithelial-to-mesenchymal transition through β-catenin/Erk/Akt signaling in gastric cancer

doi: 10.1186/s13046-018-0876-2

Figure Lengend Snippet: CHI3L1 binds to CD44 which also interacts with IL-13Rα2. a Western blot analysis of CHI3L1, CD44 and IL-13Rα2 protein expression in various gastric cancer cell lines. b - c Lysates from AGS and MGC803 cells were immunoprecipitated (IP) with control IgG and anti-CD44 or anti-CHI3L1 antibody, and then immunoblotted as indicated. Five percent of total cell lysates were used for the input. d Co-localization of CD44 (green) and CHI3L1 (red) in AGS (upper panel), MGC803 (middle panel) and GC tissues from patient #1 (lower panel) by immunofluorescent confocal microscopy (Magnification: 630×). Scale bars represent 10 μm. e - f Lysates from AGS and MGC803 cells were immunoprecipitated (IP) with IgG and anti-CD44 or anti-IL-13Rα2 antibody, and then immunoblotted as indicated. Five percent of total cell lysates were used for the input. g Co-localization of CD44 (green) and IL-13Rα2 (red) in AGS (upper panel), MGC803 (middle panel) and GC tissues from patient #2 (lower panel) by immunofluorescent confocal microscopy (Magnification: 630×). Scale bars represent 10 μm

Article Snippet: Human serum CHI3L1 levels (R&D Systems; Cat. #DC3 L10) and total (R&D Systems; Cat. #MB100B) and active (Biolegend, San Diego, CA; Cat. #437707) mouse TGF-β1 levels in mouse broncho-alveolar lavage fluid were measured using ELISA kits as directed by the manufacturer.

Techniques: Western Blot, Expressing, Immunoprecipitation, Control, Confocal Microscopy

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: Chitinase 3-like 1-CD44 interaction promotes metastasis and epithelial-to-mesenchymal transition through β-catenin/Erk/Akt signaling in gastric cancer

doi: 10.1186/s13046-018-0876-2

Figure Lengend Snippet: CHI3L1 triggers Erk and Akt signaling through CD44. a and b Western blot analysis of Erk and Akt activation in the AGS and MGC803 cells which were pre-exposed with the control antibody or functional CD44 blocking antibody and treated with rhCHI3L1 (500 ng/ml) for the indicated times. c and d Western blot analysis of CD44 and IL-13Rα2 knockdown efficacy in AGS ( c ) and MGC803 ( d ) transfected with scramble, CD44 or IL-13Rα2 shRNA. e and f Westen blot evaluation of the activation of Erk and Akt in AGS and MGC803 cells which stably expressing scrambled, CD44 or IL-13Rα2 shRNA and were cultured in the presence or absence of rhCHI3L1 (500 ng/ml). g Westen blot analysis of the activation of Erk and Akt in bone marrow-derived macrophages (BMDM) from WT and CD44 −/− mice which were incubated with the recombinant mouse CHI3L1 (500 ng/ml) for the noted periods of time

Article Snippet: Human serum CHI3L1 levels (R&D Systems; Cat. #DC3 L10) and total (R&D Systems; Cat. #MB100B) and active (Biolegend, San Diego, CA; Cat. #437707) mouse TGF-β1 levels in mouse broncho-alveolar lavage fluid were measured using ELISA kits as directed by the manufacturer.

Techniques: Western Blot, Activation Assay, Control, Functional Assay, Blocking Assay, Knockdown, Transfection, shRNA, Stable Transfection, Expressing, Cell Culture, Derivative Assay, Incubation, Recombinant

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: Chitinase 3-like 1-CD44 interaction promotes metastasis and epithelial-to-mesenchymal transition through β-catenin/Erk/Akt signaling in gastric cancer

doi: 10.1186/s13046-018-0876-2

Figure Lengend Snippet: CHI3L1 regulates β-Catenin signaling via CD44. a Western blot analyses were used to evaluate β-catenin phosphorylation (Ser675 or Ser552), and active-β-Catenin (ABC) after exposure to rhCHI3L1 (500 ng/ml) in the presence of control or CD44 neutralizing antibody (10 μg/ml) for the noted periods of time. b - d AGS cell were pretreated with control IgG or CD44 blocking antibody (10 μg/ml). Then, the immunofluorescence assays of p-β-catenin (Ser552 or Ser675) and active-β-catenin (ABC) (all in red) were performed in AGS cell treated with rhCHI3L1 (500 ng/ml). DAPI (blue) was used as a nuclear counterstain. The quantification of nuclear β-catenin positive staining in at least 200 counted cells was presented as percentage ± SEM. Magnification: 400×, Scale bars represent 20 μm. Results shown here are the representative of three independent experiments. Statistical significance was calculated using ANOVA ( b - d ). * p < 0.05

Article Snippet: Human serum CHI3L1 levels (R&D Systems; Cat. #DC3 L10) and total (R&D Systems; Cat. #MB100B) and active (Biolegend, San Diego, CA; Cat. #437707) mouse TGF-β1 levels in mouse broncho-alveolar lavage fluid were measured using ELISA kits as directed by the manufacturer.

Techniques: Western Blot, Phospho-proteomics, Control, Blocking Assay, Immunofluorescence, Staining

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: Chitinase 3-like 1-CD44 interaction promotes metastasis and epithelial-to-mesenchymal transition through β-catenin/Erk/Akt signaling in gastric cancer

doi: 10.1186/s13046-018-0876-2

Figure Lengend Snippet: Roles of CHI3L1 in promoting GC growth and metastasis in vitro. a The effects of rhCHI3L1 on AGS and MGC803 cells proliferation were measured by CCK8 assay. Data are presented as mean ± SEM. b Colony formation assays were performed for AGS and SGC7901 cells. c and d Proliferation of AGS ( c ) and MGC803 ( d ) cells were evaluated by EdU incorporation assay. e The invasion assay of AGS and MGC803 cells. f AGS cells transfected with scramble control or shRNAs targeting CD44 were subjected to wound closure assays. g GC cells were incubated with rhCHI3L1for the noted periods of time. Protein levels of Vimentin and Snail were analyzed by Western blot. CHI3L1 (500 ng/ml) in combination with control or functional CD44 neutralizing antibody (10 μg/ml) were used in the experiments. Results shown here are the representative of three independent experiments. Scale bars represent 100 μm. Statistical significance was calculated using ANOVA ( a - f ). * p < 0.05

Article Snippet: Human serum CHI3L1 levels (R&D Systems; Cat. #DC3 L10) and total (R&D Systems; Cat. #MB100B) and active (Biolegend, San Diego, CA; Cat. #437707) mouse TGF-β1 levels in mouse broncho-alveolar lavage fluid were measured using ELISA kits as directed by the manufacturer.

Techniques: In Vitro, CCK-8 Assay, Invasion Assay, Transfection, Control, Incubation, Western Blot, Functional Assay

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: Chitinase 3-like 1-CD44 interaction promotes metastasis and epithelial-to-mesenchymal transition through β-catenin/Erk/Akt signaling in gastric cancer

doi: 10.1186/s13046-018-0876-2

Figure Lengend Snippet: CD44v3 physically interacts with CHI3L1 and IL-13Rα2. a Illustration of CD44 gene and alternative spliced variants (e.g., CD44s, CD44v3, v6 and v9 isoforms) which contain external, transmembrane (TM) and intracellular domain. b The structure of CD44v3 transmembrane protein, which contains the hyaluronic acid (HA) binding sites at the external N-terminal region, a heparin sulfate (HS) assembly site in v3 domain, and the signaling regulator binding sites at the cytoplasmic region. CD44v3 domain amino acid sequences and v3 peptide used in the current study (in red) were listed. c - e Binding of CD44v3 extracellular domain (ECD) ( c ) or CD44v3 peptide ( d and e ) to rhCHI3L1 or rhIL-13Rα2 ECD. The binding affinity was evaluated by the absorbance at 450 nm in a direct ELISA. Results shown are representative of a minimum of three independent experiments. The values represent the mean ± SEM in triplicate; *p < 0.05. f and g Measurement of the binding affinity of CD44v3 peptide to rhCHI3L1 ( f ) or rhIL-13Rα2 ECD ( g ) by biolayer interferometry (BLI). Various concentrations of CD44v3 peptide were shown. All experiments were performed in triplicate. h Binding of CD44v6 peptide to rhCHI3L1 or rhIL-13Rα2 ECD evaluated by a direct ELISA as describe above. i - j Measurement of the binding affinity of CD44s ECD to rhCHI3L1 ( i ) or rhIL-13Rα2 ECD ( j ) by biolayer interferometry (BLI). CD44s ECD was immobilized and CHI3L1 (500 μM) or IL-13Rα2 (500 μM) was in the mobile phase

Article Snippet: Human serum CHI3L1 levels (R&D Systems; Cat. #DC3 L10) and total (R&D Systems; Cat. #MB100B) and active (Biolegend, San Diego, CA; Cat. #437707) mouse TGF-β1 levels in mouse broncho-alveolar lavage fluid were measured using ELISA kits as directed by the manufacturer.

Techniques: Binding Assay, Direct ELISA

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: Chitinase 3-like 1-CD44 interaction promotes metastasis and epithelial-to-mesenchymal transition through β-catenin/Erk/Akt signaling in gastric cancer

doi: 10.1186/s13046-018-0876-2

Figure Lengend Snippet: CHI3L1 mediated tumorigenesis through CD44 in vivo. a A representative image of tumor growth in nude mice subcutaneously inoculated with lenti-shCHI3L1- or lenti-shControl-transfected MGC803 cells. b The growth curve of subcutaneous tumor from MGC803 cells infected with lenti-shCHI3L1 or lenti-shControl in nude mice ( n = 10 animals per group). c Comparison of tumor weight from two groups at the end of the experiment. d Evaluation of cell proliferative activity by Ki-67 staining in subcutaneous xenografts from MGC803 cells infected with lenti-shControl or lenti-shCHI3L1. e and f H&E staining of the representative SGC7901 cells lung metastatic lesions in nude mice ( e ). The total area of invasive lesions on the lung slice section represents the invasive tumor volume in the lungs ( f ). Sizing bar indicates 100 μm. g Representative photograph of lungs from WT and CD44 −/− mice 2 weeks after challenge with B16-F10 melanoma cells. Comparison of the number of pleural melanoma colonies in lungs from tumor cells challenged WT and CD44 −/− mice ( n = 8 animals per group). h and i The levels of total ( h ) and active ( i ) TGF-β1 in broncho-alveolar lavage (BAL) fluids from WT mice and CD44 −/− mice. j Schematic representation of the CHI3L1/CD44-IL-13Rα2 signaling pathway in promoting GC cell metastasis. Data are presented as mean ± SEM, * p < 0.05 (Student’s t test)

Article Snippet: Human serum CHI3L1 levels (R&D Systems; Cat. #DC3 L10) and total (R&D Systems; Cat. #MB100B) and active (Biolegend, San Diego, CA; Cat. #437707) mouse TGF-β1 levels in mouse broncho-alveolar lavage fluid were measured using ELISA kits as directed by the manufacturer.

Techniques: In Vivo, Transfection, Infection, Comparison, Activity Assay, Staining

Journal: The European respiratory journal

Article Title: Characterization of Asthma Subgroups Associated with Circulating YKL-40 Levels

doi: 10.1183/13993003.00800-2017

Figure Lengend Snippet: Serum YKL-40 Levels in the four YKL-40 asthma clusters. A: Serum YKL-40 levels in clusters C1–C4 in the YCAAD cohort. C3 and C4 had elevated serum levels of YKL-40 compared to C1 and C2. Serum YKL-40 levels were similar among the clusters in the SARP and NYUBAR cohorts (panels B and C, respectively) compared to the YCAAD cohort. Panel D shows the combined values for all cohorts (*p<0.05; **p<0.01).

Article Snippet: Measurement of serum and sputum YKL-40 levels YKL-40 levels were measured in duplicate in serum (all cohorts) and sputum (YCAAD only) supernatant specimens by enzyme-linked immunosorbent assay (ELISA) (Quidel, San Diego, CA) as previously described. ( 1 , 11 ) Sputum induction and gene expression measurements Sputum induction was performed in the YCAAD cohort only; additional details are described in the supplement .

Techniques:

Journal: The European respiratory journal

Article Title: Characterization of Asthma Subgroups Associated with Circulating YKL-40 Levels

doi: 10.1183/13993003.00800-2017

Figure Lengend Snippet: Differences in lung function among the YKL-40 clusters in the 3 cohorts. Post-bronchodilator FEV1 was significantly different among the YKL-40 clusters in all three cohorts (panels A–C). Cluster C3 had severely reduced post-BD FEV1 in all three cohorts (**p<0.01).

Article Snippet: Measurement of serum and sputum YKL-40 levels YKL-40 levels were measured in duplicate in serum (all cohorts) and sputum (YCAAD only) supernatant specimens by enzyme-linked immunosorbent assay (ELISA) (Quidel, San Diego, CA) as previously described. ( 1 , 11 ) Sputum induction and gene expression measurements Sputum induction was performed in the YCAAD cohort only; additional details are described in the supplement .

Techniques:

Journal: The European respiratory journal

Article Title: Characterization of Asthma Subgroups Associated with Circulating YKL-40 Levels

doi: 10.1183/13993003.00800-2017

Figure Lengend Snippet: Rates of near-fatal asthma exacerbations requiring mechanical ventilation in the YKL-40 clusters among the three cohorts. The rate was significantly higher in cluster C3 in all three cohorts (*p<0.01).

Article Snippet: Measurement of serum and sputum YKL-40 levels YKL-40 levels were measured in duplicate in serum (all cohorts) and sputum (YCAAD only) supernatant specimens by enzyme-linked immunosorbent assay (ELISA) (Quidel, San Diego, CA) as previously described. ( 1 , 11 ) Sputum induction and gene expression measurements Sputum induction was performed in the YCAAD cohort only; additional details are described in the supplement .

Techniques:

Journal: The European respiratory journal

Article Title: Characterization of Asthma Subgroups Associated with Circulating YKL-40 Levels

doi: 10.1183/13993003.00800-2017

Figure Lengend Snippet: Sputum neutrophils (A) and sputum YKL-40 levels (B) in the YCAAD cohort. Both values were increased in C1 and C2 compared to C3 and C4.

Article Snippet: Measurement of serum and sputum YKL-40 levels YKL-40 levels were measured in duplicate in serum (all cohorts) and sputum (YCAAD only) supernatant specimens by enzyme-linked immunosorbent assay (ELISA) (Quidel, San Diego, CA) as previously described. ( 1 , 11 ) Sputum induction and gene expression measurements Sputum induction was performed in the YCAAD cohort only; additional details are described in the supplement .

Techniques:

Journal: The European respiratory journal

Article Title: Characterization of Asthma Subgroups Associated with Circulating YKL-40 Levels

doi: 10.1183/13993003.00800-2017

Figure Lengend Snippet: Analysis of the sputum transcriptome in the YKL-40 clusters and healthy controls in the YCAAD cohort. A: Mean T2 gene expression levels (IL-4, -5, and -13) among the YKL-40 clusters demonstrate lower levels of T2 gene expression in C3 and C1 and C4. B: Heatmap of the sputum gene expression in C3 compared to healthy controls (HC) shows activation of innate immunity pathways and the NETosis pathway. C: Heatmap of the sputum gene expression in C3 compared to HC shows activation of the IL-1 and IL-18 pathways in C4.

Article Snippet: Measurement of serum and sputum YKL-40 levels YKL-40 levels were measured in duplicate in serum (all cohorts) and sputum (YCAAD only) supernatant specimens by enzyme-linked immunosorbent assay (ELISA) (Quidel, San Diego, CA) as previously described. ( 1 , 11 ) Sputum induction and gene expression measurements Sputum induction was performed in the YCAAD cohort only; additional details are described in the supplement .

Techniques: Expressing, Activation Assay

Journal: Annals of Translational Medicine

Article Title: Clinical correlation between serum YKL-40 protein level and recurrence of non-muscle invasive bladder cancer

doi: 10.3978/j.issn.2305-5839.2015.11.22

Figure Lengend Snippet: Table 1

Article Snippet: Serum YKL-40 level was determined using human cartilage glycoprotein-39 ELISA kit (Quidel, USA) in strict accordance with the manufacturer’s instructions.

Techniques: